小鼠A20鼠B细胞淋巴瘤的全身和皮下建模:比较评估

作者:

Sheri R. Barnes, Sumithra Urs, David Draper, Scott Wise, and Maryland Rosenfeld Franklin

年份:

2019

美国癌症研究协会(AACR)年会

 

简介与背景

  • 随着肿瘤学领域对免疫系统靶向分子的需求增加,我们需要可靠且特征明确的临床前模型。
  • Orthotopic modeling is becoming increasingly employed in preclinical development as it is posited to have improved clinical translatability compared to subcutaneous implantation.
  • We developed a luciferase enabled A20 murine B cell lymphoma cell line (A20-luc) to better understand the utility of subcutaneous and orthotopic modeling of A20 in preclinical oncology drug development.
  • A panel of checkpoint inhibitors and costimulatory agonists were assessed for anti-tumor response both in the subcutaneous and systemic disease settings.
  • Investigation of infiltration of lymphoid and myeloid cell subsets in subcutaneous A20 tumors were evaluated. Knowledge of this immune composition can help guide rational monotherapy and combination strategies.